Bacterial infection can trigger multiple Pattern Recognition Receptors (PRRs), in a pathogen dependent manner, to trigger inflammatory responses to controls and clear infection. How PRRs signal and how multiple receptor inputs are integrated into the host response to infection are unclear. Visualising how individual PRRs signal and the structure of their endogenous signaling platforms is helping to elucidate these mechanisms. The activity of PRRs is also tightly regulated to prevent excessive triggering of inflammation. The mechanisms for regulation of PRR signaling and the physiological consequences when this process is disrupted are unclear. We use high resolution microscopy, in vitro cell biology analysis and in vivo studies to investigate how PRR signaling impacts on the host response to Salmonella infection.