Poster Presentation Lorne Infection and Immunity 2018

Murine cytomegalovirus degrades MHC class II to colonize the salivary glands     (#149)

Joseph Yunis 1 , Helen E Farrell 1 , Kimberely Bruce 1 , Clara Lawler 1 , Stine Sidenius 1 , Orry Wyer 1 , Nick Davis-Poynter 1 2 , Philip G Stevenson 1 2
  1. School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, Australia
  2. Centre for Children's Health Research, South Brisbane, QLD, Australia

Cytomegaloviruses (CMVs) establish persistent, systemic infections of immunocompetent hosts. Their evasion of CD8+ T cells is well known. The myeloid cells that CMVs colonize can also potentially interact with CD4+ T cells via MHC class II (MHC II). Human CMV attacks the MHC II presentation pathway in vitro, but how CD4+ T cell evasion contributes to host colonization is unknown. CMV infections long pre-date the divergence of primates from rodents, and Murine CMV (MCMV) is likely to have a similar need for evasion. It down-regulated MHC II via M78, a 7 transmembrane domain viral protein that captured MHC II from the cell surface for degradation in lysosomes. M78-deficient MCMV failed to down-regulate MHC II, and after nasal inoculation showed a defect in salivary gland colonization that was significantly rescued by CD4+ T cell loss and anti-interferon gamma treatment. Therefore MCMV requires CD4+ T cell evasion which restricts interferon gamma response to colonize its main site of long-term shedding.